PAL3-TH-D857-uSPE
Automated Parallel Derivatization of Metabolites
For metabolite profiling chemical derivatization has been used to improve MS sensitivity and LC retention. However, for multi-analytes quantification, the number of available isotopically labelled internal standards is limited. And, there is no single workflow which can provide large-scale metabolomics coverage in particular for polar metabolites.
Presented by:
Prof. Gerard Hopfgartner, Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva
Streamlining Metabolomic Analyses with Automated Derivatization and Injection Workflows
Metabolites, the dynamic molecules reflecting cellular processes, hold the potential for understanding health, disease, and
biological systems. To unlock this potential, metabolomic analyses require precise and reliable analytical methods. This App Note
explores the workflow of the Metabolomics Core Technology Platform (MCTP), a core facility of Heidelberg University (Germany),
discussing their semi-automated analysis for untargeted metabolomics and ¹³C tracing studies via gas chromatography-mass
spectrometry (GC/MS).
Automated Workflow for the determintation of Fatty Acid Methyl Esters (FAME)
This work presents a fully automated workflow using a workstation with robotic tool change (RTC based on a method using sodium methoxide in methanol as reactant .The workflow improves process safety, optimizes throughput and minimizes handling error.
