Micro-SPE Tool and Rack with Micro SPE Cartridges on a PAL System

Micro-SPE Cartridges

PAL Micro-SPE Cartridges

PAL micro-SPE cartridges stand for a miniaturized and robust SPE technology that allows reliable automation and reduction of used sample and solvent without compromising performance. The uSPE has therefore emerged as a new standard for extraction, purification, concentration or filtration for automated environmentally friendly analysis processes.

 

PAL Micro SPE Cartridge explosion drawing

The new PAL micro-SPE Cartridge consists of two parts. The polymer material used is chemically inert and free from leachables. The outer part (1) provides a higher capacity and flexible volume for filter disks (3) and the sorbent material (4). The bottom outlet is designed to penetrate pre-slit septa and to deliver directly to LC injection ports. The inner plunger (2) provides critical functionality with the compression of the sorbent/filter layers, and a precise needle guide for safe and upright transport. An essential feature is the ability to withstand high pressure and prevent leakage around the syringe needle, which allows for faster loading of larger sample volumes. Applications range from food pesticides, water, and drug analysis to forensic and pharmacological applications.

Micro SPE Cartridge and uSPE Tool

Key functionality of micro-SPE Cartridges:

  • Septumless cartridge designs with patented sealing
  • High pressure rating (up to 10 bar)
  • Multiple elutions supported
  • Sorbent capacity: up to 100 mg packing possible
  • Chemically inert materials used 
  • Free of extractables & leachables
  • Designed to penetrate pre-slit septa of vials
  • Designed to elute directly into PAL System liquid injection ports

PAL micro-SPE Cartridge Selection Guide with Publications

Cartridges

 

Application Information

 

Publications

uSPE-GCQuE1-45-V 
PAL System µSPE Cartridge
GC QuEChERS sorbent mix 1, 45 mg​
[C18, PSA, MgSO4, GCB]
108 pcs type 01-05B

 

µSPE Cartridge for GC amenable pesticides.  
Follows AOAC 2007.01 and EN 15662 methodology.

 

Steven Lehotay et al, 2022​
https://doi.org/10.1016/j.chroma.2022.463596 

Amadeo Fernandez-Alba et al. 2023, open Access
https://doi.org/10.1016/j.chroma.2023.463906

Mette Poulson et al., 2021​, open Access
https://doi.org/10.1016/j.chroma.2021.462384

Fully Automated QuEChERS Extraction and Clean-up of Organophosphate
Pesticides in Orange Juice, Hübschmann et al, 2023

Coupling micro-flow liquid chromatography with Q-Orbitrap high-resolution mass spectrometry for greener, comprehensive pesticide residue analysis in fruits and vegetables 
Felicia Jesus, 2026
https://doi.org/10.1016/j.foodchem.2026.148447

Performance Qualification Test  for PAL System uSPE GC QuEChERS-45-V  Cartridges 

uSPE-GCQuE2-44-V 
PAL System µSPE Cartridge
GC QuEChERS sorbent mix 2, 44 mg​
[C18, PSA, MgSO4]
108 pcs type 01-19A

 

µSPE Cartridge without Carbon for GC amenable pesticides.
Follows AOAC 2007.01 and EN 15662 methodology.

 

Steven Lehotay et al, 2022​
https://doi.org/10.1016/j.chroma.2022.463596 

Amadeo Fernandez-Alba et al. 2023, open Access
https://doi.org/10.1016/j.chroma.2023.463906

Mette Poulson et al., 2021​, open Access
https://doi.org/10.1016/j.chroma.2021.462384

Fully Automated QuEChERS Extraction and Clean-up of Organophosphate
Pesticides in Orange Juice, Hübschmann et al, 2023

Performance Qualification Test  for PAL System µSPE GC QuEChERS-45-V  Cartridges 

uSPE-GCQuE3-45-V
PAL System µSPE Cartridge
GC QuEChERS sorbent mix 3, 45 mg​
[PSA, MgSO4]
108 pcs type 01-14A

 

µSPE Cartridge for GC amenable pesticides in low fat matrices.
Follows AOAC 2007.01 and EN 15662 methodology.

 

 

uSPE-LC-QuE1-30-T 
PAL System μSPE Cartridge
LC & GC QuEChERS sorbent mix, 30 mg​
[C18, Z-Sep, GCB]
96 pcs in blister type 01-03A

 

µSPE Cartridge for pesticides in complex spice matrixes

 

Goon et al: Journal of AOAC International Vol. 103, No.1, 202
https://doi.org/10.5740/jaoacint.19-0202

Performance Qualification Test  for PAL System uSPE LC QuEChERS-30-T  Cartridges 

uSPE-SAX-50-V
PAL System µSPE Cartridge
Strong Anion Exchange (SAX), 50 mg​
for polar, anionic pesticides or other anionic compounds
108 pcs type 01-20A

 

µSPE Cartridge for LC amenable pesticides.
ollows AOAC 2007.01 and EN 15662 methodology. 

 

Analysis of Highly Polar Pesticides  - Automated Micro-SPE Clean-up of Complex Food Matrices for LC-MS/MS
Florencia Jesús , Amadeo Rodríguez Fernández-Alba, Hans-Joachim Hübschmann

uSPE-WAX-50-V
PAL System µSPE Cartridge WAX, 50 mg
108 pcs type 01-21A

 

uSPE Cartridge for PFAS analysis

 

ASMS 2025 Poster, Duke University 
Fully automated implementation of EPA Method 1633 for LC-MS/MS analysis of PFAS in environmental samples using a customized robotic autosampler

Aghogho A. Olomukoro et al, 2025​, Open Access
https://doi.org/10.1007/s00216-025-05908-x
Evaluation of extraction methodologies for PFAS analysis in mascara: comparative study of SPME and automated μSPE

uSPE-GCB-10-V
PAL System µSPE Cartridge GCB, 10 mg
108 pcs type 01-22A

 

µSPE Cartridge for PFAS analysis

 

ASMS 2025 Poster, Duke University 
Fully automated implementation of EPA Method 1633 for LC-MS/MS analysis of PFAS in environmental samples using a customized robotic autosampler

Aghogho A. Olomukoro et al, 2025​, Open Access
https://doi.org/10.1007/s00216-025-05908-x
Evaluation of extraction methodologies for PFAS analysis in mascara: comparative study of SPME and automated μSPE

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