蛋白质组学研究、癌症和生物标记物研究 等生命科学应用的自动化非常有价值。典 型的是来自需要高度可重复处理的患者队 列的大样本系列，这是PAL System的强 项。
可温度控制的样品储存、重叠和提前样品 制备，确保每个样品都以完全相同的方式 处理，以实现数据的良好再现性和可比 性。PAL System的无人值守、全自动操作 已整合到所有领先的色谱质谱制造商的色 谱软件数据系统中。
PAL System通过高效工作流程的专用工具和 模块，使临床、生物技术和生物医学研究 中广泛的样品制备应用自动化并用于在线 LC-MS和GC-MS分析。应用包括Bligh&Dyer 法、液液萃取、蛋白质沉淀和离心等。对 于代谢组学分析，高灵敏度分析需要分析 物的衍生化。
对于治疗药物监测，专用的PAL LC-MS 工具提供从初级管直接采样。光学传感 器位于工具的样品环上，用于监测血液 或血清样本抽吸时的气隙或气泡。
在诊断和癌症生物标志物研究的临床应 用中，对挥发物进行呼吸气体分析是使 用坚固的固相微萃取（SPME Arrow）工具是另一个快速发展的领域。
PAL System通过阐明蛋白质在活性或非 活性状态下的三维结构来支持对蛋白质 生物活性的研究。氢氘交换（HDX）是 一种标记技术，可以研究蛋白质链在生 理条件下与结合伴侣相互作用时如何折 叠。
Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples
Keywords: phosphoproteome, fractionation, 2D LC
Anal. Chem. 2019 91 (15), 9707-9715
Metabolomic signature of brain cancer
Keywords: adult glioblastoma; magnetic resonance spectroscopy; mass spectrometry; metabolomics; paediatric glioblastom; GC-MS, ITEX Dynamic Headspace
Mol. Carcinog. 2017 Jun 15. doi: 10.1002/mc.22694. [Epub ahead of print]
Compound Property Optimization in Drug Discovery Using Quantitative Surface Sampling Micro Liquid Chromatography with Tandem Mass Spectrometry
Keywords: surface sampling, LC-MS, quantitative tissue distribution, drug discovery
Anal Chem, 2016, 88, 11813-20
Development of a Biocompatible In-Tube Solid-Phase Microextraction Device: A Sensitive Approach for Direct Analysis of Single Drops of Complex Matrixes
Keywords: SPME, biocompatible, complex matrices, in-tube, single drop
Anal Chem, 2016, DOI: 10.1021/acs.analchem.6b03160
Unbiased profiling of volatile organic compounds in the headspace of Allium plants using an in-tube extraction device (OPEN ACCESS)
Keywords: Volatile organic compounds, VOC, GC-TOF–MS, Metabolomics, Headspace, ITEX, Allium
BMC Res Notes, 2016, 9, 133
Proteomics of Cerebrospinal Fluid: Throughput and Robustness Using a Scalable Automated Analysis Pipeline for Biomarker Discovery
Keywords: proteomics, cerebrospinal fluid, CSF, throughput, robustness, RP-LC MS/MS
Anal Chem 2015, 87, 10755-10761
Extended Coverage of Singly and Multiply Phosphorylated Peptides from a Single Titanium Dioxide Microcolumn
Keywords: proteomics, coverage phosphorylated peptides, phospho petides, titanium dioxide microcolum
Anal Chem 2015, 87, 10213-2
An enhanced droplet-based liquid microjunction surface sampling system coupled with HPLC-ESI-MS/MS for spatially resolved
Keywords: droplet-based liquid microjunction surface sampling, complex matrix samples, chemical information from single spot
Anal Bioanal Chem, 2015, 407, 2117-25
Organic Acid Quantitation by NeuCode Methylamidation
Keywords: multiplexed quantitative analysis method, carboxylic acids, liquid chromatography high resolution mass spectrometry, LC-MS
Anal Chem, 2014, 86, 4402-4408
Time Window Expansion for HDX Analysis of an Intrinsically Disordered Protein.
J Am Soc Mass Spectrom 2013; 24(10): 1584-92
Quantitative Metabolite Profiling Utilizing Parallel Column Analysis for Simultaneous Reversed-Phase and Hydrophilic Interaction Liquid Chromatography Separations Combined with Tandem Mass Spectrometry
Keywords: metabolomics, profiling, orthogonal separation, column switching
Anal Chem, 2014, 86, 4145-4150
Method Development for Fecal Lipidomics Profiling (OPEN ACCESS)
Keywords: Fecal analysis, lipidomics, mass spectrometry, liquid chormatography, high pressure cycling LC/MS, premature infant, neonate
Anal Chem, 2013, 85, 1114−1123
Evaluation of a novel commercial assay for the determination of cyclosporine A, tacrolimus, sirolimus and everolimus by liquid chromatography-tandem mass spectrometric assay
Keywords: immunosuppressant, blood samples, in-vitro diagnostic (IVD)- mass spectrometric immunosuppressant assay, LC/MS
Ther Drug Monit, 2013, 35, 129-32
Rapid Screening for Potential Epitopes Reactive with a Polycolonal Antibody by Solution-Phase H/D Exchange Monitored by FT-ICR Mass Spectrometry
J. Am. Soc. Mass Spectrom. (2013) 24;1016-1025
Granger causality in integrated GC–MS and LC–MS metabolomics data reveals the interface of primary and secondary metabolism
Metabolomics (2013) 9: 564–574
Improved Protein Hydrogen/Deuterium Exchange Mass Spectrometry Platform with Fully Automated Data Processing
Anal. Chem. 2012, 84, 4942−4949
Determination of paracetamol in mouse, rat and dog plasma samples by laser diode thermal desorption–APCI-MS/MS
Bioanalysis (2012) 4(11), 1327–1335
Proteomic Profiling of Thyroid Papillary Carcinoma
J Thyroid Res. 2012;2012:815079
High-throughput flow injection analysis of labeled peptides in cellular samples – ICP-MS analysis versus fluorescence based detection
Int J Mass Spectrom. 2011, 307, 105–111
Methods for the Analysis of High Precision Differential Hydrogen Deuterium Exchange Data
Int J Mass Spectrom. 2011; 302(1-3): 59–68
Drug-excipient compatibility testing—Identification and characterization of degradation products of phenylephrine in several pharmaceutical formulations against the common cold
J Pharm Biomed Anal 55 (2011) 949–956
Structural characterization of both the non-proteolytic and proteolytic activation pathways of coagulation Factor XIII studied by hydrogen–deuterium exchange mass spectrometry
Int. J. Mass Spec, 302, 2011, 139–148
Strategy of Accelerated Method Development for High-Throughput Bioanalytical Assays Using Ultra High-Performance Liquid Chromatography Coupled with Mass Spectrometry
Anal. Chem. 2009, 81, 9225–9232
Comprehensive Analytical Strategy for Biomarker Identification based on Liquid Chromatography Coupled to Mass Spectrometry and New Candidate Confirmation Tools
Anal. Chem. 2009, 81, 7677–7694
TopCount coupled to ultra-performance liquid chromatography for the profiling of radiolabeled drug metabolites in complex biological samples
Keywords: Scintillation counter, radiolabeling, UHPLC
J Chromatogr B Analyt Technol Biomed Life Sci. 2006; 844(1):96-103
Cherry-picking in an orchard: unattended LC/MS analysis from an autosampler with > 32,000 samples online
Keywords: High throughput screening, LC/MS
J Biomol Screen. 2006 April,11 (3): 318-22. EPub 2006 Feb 20.
Enantiomeric separation and quantification of pindolol in human plasma by chiral liquid chromatography/tandem mass spectrometry using staggered injection with a CTC Trio Valve System
Keywords: High throughput screening, LC/MS
Rapid Commun Mass Spectrom. 2006; 20(2): 291-7
Description and Validation of a Four-Channel Staggered LC-MS/MS System for High-Throughput in Vitro Screens
Keywords: High throughput Screening, LC/MS
Anal. Chem. 2005, 77, 1905-1910
SpeedScreen: The “Missing Link” between Genomics and Lead Discovery
Keywords: High throughput screening, LC/MS
J Biomolecular Screening 2004: 9, 498-505
Headspace solid-phase microextraction and gas chromatography-mass spectrometry for the determination of 5alpha-androst-2-en-17-one and -17beta-ol in the female Asian elephant: application for reproductive monitoring and prediction of parturition
Keywords: SPME, GC-MS
J Steroid Biochem Mol Biol. 2003 Feb; 81(2-3):383-91
Derivatization of Ehinylestradiol with Dansyl Chloride To Enhance Electrospray Ionization: Application in Trace Analysis of Ethyinylestradiol in Rhesus Monkey Plasma
Keywords: Ethinylestradiol, derivatization dansyl chloride, LC/MS
Anal Chem. 2002, 74(16), 4136-44
Automated Determination of Drugs of Abuse in Hair Samples
Hair testing is a powerful tool routinely used for the detection of drugs of abuse in forensic and toxicology applications.
Automated Parallel Derivatization Strategy with Broad Metabolite Coverage Coupled to SWATH/MS Data Acquisition for Qualitative and Quantitative Analysis
Due to the high dynamic range of different metabolites, SWATH/MS data acqusition allows the quatification on both precursor and product ion level including flexibility of the quantifier choice.
PAL LC-MS Tools
Three new LC-MS Tools, all equipped with Smart Syringes.
Automated Sample Prep Workflows: Save money and time
This compendium on instrumental sample preparation showcases the many proven solutions of automated sample preparation workflows for food, environmental, pharma, life science analysis, and beyond.
Static and Dynamic Headspace Analysis
The PAL Compendium
PAL Ingenious News January 2022
PAL Ingenious News June 2022
Analysis of Metabolites by LC-MALDI-MS/MS
In the present work were evaluated the use of UHPLC-MALDI-MS/MS for the analysis of metabolites of talinol generated in-vitro.
Automated in-gel digestion on a commercial autosampler directly coupled to nanoLC-MS/MS
We have adapted a manual in-gel digestion protocol, including reduction, alkylation and acid-labile detergent assisted trypsin digestion of proteins, to be performed on a PAL RTC autosampler system.
Automated Parallel Derivatization of Metabolites
For metabolite profiling chemical derivatization has been used to improve MS sensitivity and LC retention. However, for multi-analytes quantification, the number of available isotopically labelled internal standards is limited. And, there is no single workflow which can provide large-scale metabolomics coverage in particular for polar metabolites.
Presented by:
Prof. Gerard Hopfgartner, Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva
Challenges and Opportunities of Sample Preparation for Complex Matrices in Liquid Chromatography coupled with Mass Spectrometer
This presentation focuses on Sample Preparation aspecting in Metabolomics and Lipidomics
Presented by:
David Ruskic and Prof. Gerard Hopfgartner, Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva
