A single-sample workflow for joint metabolomic and proteomic analysis ofclinical specimens
Integrating proteomic and metabolomic data enhances ourunderstanding of biological processes and diseasemechanisms. Traditional multi-omics workflows often requireseparate sample processing, leading to increased variability.To address this, we developed MTBE-SP3, an optimizedsingle-sample workflow that combines a 75% EtOH/MTBEmetabolite extraction with automated single-pot solid-phase-enhanced sample preparation for proteins.