Automated Parallel Derivatization of Metabolites
For metabolite profiling chemical derivatization has been used to improve MS sensitivity and LC retention. However, for multi-analytes quantification, the number of available isotopically labelled internal standards is limited. And, there is no single workflow which can provide large-scale metabolomics coverage in particular for polar metabolites.
Presented by:
Prof. Gerard Hopfgartner, Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva
Automated Parallel Derivatization Strategy with Broad Metabolite Coverage Coupled to SWATH/MS Data Acquisition for Qualitative and Quantitative Analysis
Due to the high dynamic range of different metabolites, SWATH/MS data acqusition allows the quatification on both precursor and product ion level including flexibility of the quantifier choice.
Automated sample preparation in the proteomics laboratory
Automation of the digestion step provides a number of advantages, in particular for reproducibility, purity and time consumption. Unlike most other sample preparation systems the PAL RTC allows the preparation of smaller sample batches, which suites the medium-throughput requirements of a proteomic laboratory.
Automation of Hydrogen Deuterium Exchange Experiments (HDX)
HDX experiments are an example of where automation has great value. The requirement for significant investment of time in the data processing tasks places very high emphasis on obtaining the best quality experimental data. Variance and potential mistakes inherent in manual experimental technique result in wasted effort processing poor data.
LC/MS metabolomics platform with hyphenation of extraction and analysis for polar and non-polar metabolites in biological samples
The automation of the entire sample preparation process, incl. online injection on the PAL RTC achieves excellent results and high productivity. Using a mixed mode μSPE cartridge enables the efficient class separation of lipids (i.e. neutral and phospholipids).
New Biopharmaceuticals: Bispecific Antibody Clone Selection
Herein, we describe the development of a new high-throughput approach based on the newly introduced SampleStream Platform for performing clone selection screening, enabling selection of clones that produce the highest amount and quality of the correctly assembled bispecific antibody.
Metabolomics: Automated Bligh Dyer extraction of tissues and cells with online LC/MS analysis
Here we propose to integrate an automated Bligh and Dyer extraction on a robotic system including a dual-column UHPLC-MS/MS platform for the metabolomic analysis of tissues or cells. The aqueous fraction is split and analysed sequentially at two different mobile phase pH values, whereas the lipidic fraction is analysed alternately with an extended gradient.
Untargeted 2DxLC-Mass Spectrometry using SWATH-DIA-Based Workflow for the Characterization of Lipid Profiles in Plasma Samples
The use of lipidomic analyses based on LC-mass spectrometry has become widespread due to its sensitivity and specificity which provides a broader picture of lipid profiles in biofluids. In this work, we combined an automated sample extraction and a two-dimensional liquid chromatography (2DxLC) system with HILIC-based class separation followed by an orthogonal reverse-phase chromatography with an untargeted SWATH-DIA workflow to accurately annotate phosphatidylcholines (PC), lysophosphatidylcholine (LPC) and etherphosphatidylcholines (PC O-) in human plasma samples.
