在几分钟内的方法

用于 GC-MS 或 LC-MS 分析食品 QuEChERS 提取物中农药和环境污染物的自动 μSPE 净化技术

See this detailed video of the µSPE workflow for the clean-up of raw pesticides extract. The PAL µSPE Cartridge is operated in scavenger mode eluting the pesticides in a clean extract. The matrix is kept on the sorbent material. The cleaned extract can be injected without waiting time online for LC/MS or GC/MS analysis. All samples are treated on the same time axis for highest reliability.

Recent developments in pesticides analysis extend the well-established methods to higher productivity and reliability while minimizing consumption and exposition to harmful chemicals. A workflow was developed that allows automatic preparation of calibration standards, automatic sample dilution, and automatic clean-up of sample extracts on one instrument, showing that miniaturized automated solutions are reducing errors, improving analytical consistency and protecting the environment in one convenient approach.

Presented by:

Thomi Preiswerk, Senior Account Manager, CTC Analytics AG

Gwen Lim , Specialist for automated sample preparation, CTC Analytics

Ederina Ninga, Postdoc, Research Group for Analytical Food Chemistry, Danish Technical University 

The QuEChERS analysis method is a popular Solid Phase Extraction method used for the analysis of pesticide residues in food. However, there are analytical and logistical limitations during cleanup. The Webinar shows two workflow variations for high sample throughput in online GC/MS and LC/MS analysis. 

Presented by:
Hans-Joachim Huebschmann, PhD, Gwen Lim and Chiew Mei Chong, CTC Analytics AG

In this presentation we want to discuss an important development and a demand from analytical laboratories about the standardization and the automation of sample preparation procedures for food safety, environmental, the forensic or pharmaceutical analysis.

Presented by:
Hans-Joachim Huebschmann, PhD

• Learn about the importance of fundamental aspects of sample preparation and modern, sustainable sample preparation technologies
• Find out about the latest innovations in sample preparation in environmental analysis
• Learn about new materials used in sample preparation

Presented by:
Günter Boehm, PhD, VP Sample Prep Solutions, CTC Analytics AG

Chiew Mei Chong1, Hans-Joachim Hübschmann2

1 CTC Analytics Pte. Ltd., Singapore.
2 HANS Analytical Solutions, Osaka, Japan.

Micro-SPE (μSPE) emerged as an automated green micromethod for sample preparation and clean-up in food safety, proteomics, forensic, environmental and analysis since more than ten years. Applications are wideranging and cover drugs, environmental contaminants, and, in particular, the extract clean-up in multiresidue pesticide analysis. The automation of the μSPE sample preparation steps led to the desired standardization of the applied sorbent materials for extract clean-up for the large variety of food commodities, an increase in sample throughput, and the welcome potential for offline and online hyphenation with GC/MS, LC/MS or even IC analysis.

The distinctive onion and fruity odorous compounds were detected by both static headspace and ITEX-DHS techniques.

This study demonstrated the application of an automated high-throughput mini-SPE cleanup for the  analysis of pesticides and environmental contaminants in QuEChERS extracts of foods.

This note covers the development and use of miniaturized SPE for QuEChERS.

A fully automated, true green analytical method, for high sensitivity and precision pesticide analysis. This report describes for the first time a fully automated QuEChERS extraction and clean-up workflow for homogeneous matrices like fruit juices, demonstrated for orange juice.

This report presents for the first time the application of µSPE for the clean-up of the raw extracts using ethyl acetate as extraction solvent for pesticides from different also more complex food commodities.

Automated μSPE cleanup for GC/MS or LC/MS analysis of pesticides and environmental contaminants in QuEChERS extracts of foods

The automated mini-SPE clean-up workflow improved the efficiency of analysis and significantly increased the sample throughput.

The objective was to establish an automated µSPE clean-up procedure for PAH analysis in edible oils, here used sunflower oil. We were using the greater concepts of Green Analytical Chemistry and automation.

A fully automated, true green analytical method, for high sensitivity and precision pesticide analysis. This report describes for the first time a fully automated QuEChERS extraction and clean-up workflow for homogeneous matrices like fruit juices, demonstrated for orange juice.

Since more than ten years micro-SPE (µSPE) emerged as a micromethod for sample preparation and clean-up in food safety, proteomics, forensic, environmental and analysis. Applications are wide-ranging and cover drugs, environmental contaminants, and, in particular, the QuEChERS extract cleanup in multiresidue pesticide analysis. The automation of the µSPE sample preparation steps led to the desired increase in sample throughput, unification of the used sorbent materials for food commodities, and the potential for the online hyphenation with GC/MS and LC/MS instrumentation.
 

The μSPE direct injection workflow has been optimized and used for the high-throughput cleanup of liquid samples. Several μSPE cartridges are currently being tested by LCMS for the cleanup of complex sample matrices containing various pesticides and drugs, in order to further validate the performance of the direct μSPE-LCMS workflow in different use cases.

The μSPE clean-up efficiency was demonstrated in the removal of matrixinterfering components and in the recovery of pesticides.

Using PAL Robotic Tool Change (RTC) system, a fully automated QuEChERS was developed for the extraction and clean-up of organochlorine and organophosphate pesticides from homogeneous samples. The automated QuEChERS workflow includes extraction with acetonitrile, salting out with
saturated sodium chloride solution and the clean-up with PAL μSPE prior to injection into the GC-MS/MS for analysis.

In this poster presentation, we compare two workflows for the cleanup of multi-class veterinary drugs in animal tissue matrices, based on the Thermo Scientific™ VetDrugs Explorer Collection (P/N VDX-TSQ02-10001, Thermo Fisher Scientific, Waltham MA, USA).