QuEChERS(快速、简单、廉 价、有效、坚固和安全)萃取 法通常用于分析各种基质中的 农药和其他污染物。它通常包 括在盐的混合物存在下用乙腈 提取,然后离心。然后通过手 动分散固相萃取(d-SPE)净化 上清液,以去除不需要的基质 化合物。
微型SPE净化使用了取代dSPE 的小柱。它实现了可靠、高通 量的样品处理,同时减少了人 力使用和仪器维护。微型SPE 对QuEChERS提取物的净化是 在清除模式下进行的,其中不 需要的基质被微SPE小柱上的 吸附剂保留,目标化合物在快 速洗脱曲时被收集。
此外,使用PAL System 、微型 SPE小柱和工作流程也实现了 乙酸乙酯提取物的自动净化 (瑞典乙酸乙酯法,SweEt)。
对各种分析物和食物(苹果、猕猴桃、胡萝卜、羽衣甘蓝、橙子、黑橄榄、猪腰肉、三文鱼和鳄梨)取得高质量结果;Lehotay et al.,2016;
实现可靠、高通量样品处理24/7,微型SPE步骤每个样本前处理仅需要8分钟;
无需样品体积浓缩;
人力投入和仪器维护降至最低
实现比分散SPE(dSPE)更好的净化;
更好的净化,大大延长了仪器的可运行时间。
我们的合作伙伴与我们一起,已经实现了集样本准备、数据采集和分析于一体的完整解决方案:
Walk away QuEChERS extracts clean-up
See this detailed video of the µSPE workflow for the clean-up of raw pesticides extract. The PAL µSPE Cartridge is operated in scavenger mode eluting the pesticides in a clean extract. The matrix is kept on the sorbent material. The cleaned extract can be injected without waiting time online for LC/MS or GC/MS analysis. All samples are treated on the same time axis for highest reliability.
Advantages of automated and miniaturized µSPE clean-up in pesticides residues analysis
Recent developments in pesticides analysis extend the well-established methods to higher productivity and reliability while minimizing consumption and exposition to harmful chemicals. A workflow was developed that allows automatic preparation of calibration standards, automatic sample dilution, and automatic clean-up of sample extracts on one instrument, showing that miniaturized automated solutions are reducing errors, improving analytical consistency and protecting the environment in one convenient approach.
Presented by:
Thomi Preiswerk, Senior Account Manager, CTC Analytics AG
Gwen Lim , Specialist for automated sample preparation, CTC Analytics
Ederina Ninga, Postdoc, Research Group for Analytical Food Chemistry, Danish Technical University
Automated Sample Preparation for pesticides using QuEChERS extraction and cleanup
The QuEChERS analysis method is a popular Solid Phase Extraction method used for the analysis of pesticide residues in food. However, there are analytical and logistical limitations during cleanup. The Webinar shows two workflow variations for high sample throughput in online GC/MS and LC/MS analysis.
Presented by:
Hans-Joachim Huebschmann, PhD, Gwen Lim and Chiew Mei Chong, CTC Analytics AG
Fully Automated QuEChERS Extraction and µSPE Clean-up of Organophosphate Pesticides in Orange Juice
Chiew Mei Chong1, Hans-Joachim Hübschmann2
1 CTC Analytics Pte. Ltd., Singapore.
2 HANS Analytical Solutions, Osaka, Japan.
Analysis of Highly Polar Pesticides
Micro-SPE (μSPE) emerged as an automated green micromethod for sample preparation and clean-up in food safety, proteomics, forensic, environmental and analysis since more than ten years. Applications are wideranging and cover drugs, environmental contaminants, and, in particular, the extract clean-up in multiresidue pesticide analysis. The automation of the μSPE sample preparation steps led to the desired standardization of the applied sorbent materials for extract clean-up for the large variety of food commodities, an increase in sample throughput, and the welcome potential for offline and online hyphenation with GC/MS, LC/MS or even IC analysis.
Automated Mini‑Column Solid‑Phase Extraction Cleanup for High‑Throughput Analysis of Chemical Contaminants in Foods
This study demonstrated the application of an automated high-throughput mini-SPE cleanup for the analysis of pesticides and environmental contaminants in QuEChERS extracts of foods.