Name: Fully Automated Workflows for PFAS Analysis
Brand: PAL System

Optimizing the Preconcentration of Volatile PFAS Precursors - A Comparative Study of SPME Geometries

Optimizing the Preconcentration of Volatile PFAS Precursors - A Comparative Study of SPME Geometries

This Application Note summarizes a study by Williams et al. (2025) that performs a comprehensive comparison of two Solid Phase Microextraction (SPME) geometries: the traditional SPME Fiber and the robust SPME Arrow. The study systematically evaluates the impact of SPME geometry and extraction mode (Headspace vs. Direct Immersion) on extraction efficiency. The results provide a clear, data-driven basis for method development, showing how SPME configurations can be optimized for the specific physicochemical properties of different PFAS analytes.

Application Notes

Tackling Forever Chemicals

A Fully Automated Workflow for High-Throughput Analysis of Volatile PFAS in Food Contact Materials.

PFAS in Cosmetics -A Comparative Analysis of Sample Preparation Techniques for PFAS Analysis

Application Notes

PFAS in Cosmetics -A Comparative Analysis of Sample Preparation Techniques for PFAS Analysis

This application note explores a comparative study that tackles this challenge head-on by evaluating two powerful sample preparation techniques: Solid Phase Microextraction (SPME) and Micro Solid Phase Extraction (µSPE) for analyzing eight key PFAS in mascara by Liquid Chromatography with Mass Spectrometry (LC/MS). The investigation revealed that the two methods offer complementary strengths. SPME proved more sensitive for water-soluble PFAS and offered a simpler, filtration-free workflow. In contrast, automated µSPE excelled for oil-soluble PFAS but required a sample filtration step.

ASMS 2025 - Fully automated implementation of EPA Method 1633 for LC-MS/MS analysis of PFAS in environmental samples using a customized robotic autosampler

Posters

ASMS 2025 - Fully automated implementation of EPA Method 1633 for LC-MS/MS analysis of PFAS in environmental samples using a customized robotic autosampler

Widespread use and persistence of PFAS has led to ubiquitous detection in water, soil, air, and living organisms.
US EPA Method 1633 is intended for analysis of PFAS in aqueous, solid and tissue samples using LCMS/ MS at part-per-trillion concentrations.
This method involves significant sample preparation and purification steps prior to instrumental analysis, leading to bottlenecks in sample processing.
We have developed a fully automated, online μSPE-LC-MS/MS workflow based on a customized LC TriPlus RSH SMART Advanced system for high-throughput analysis of PFAS in environmental samples following EPA Method 1633 guidelines

ASMS 2025 - Fully Automated Workflow for Volatile PFAS Analysis in Food Contact Materials Using GC-Triple Quadrupole

Posters

ASMS 2025 - Fully Automated Workflow for Volatile PFAS Analysis in Food Contact Materials Using GC-Triple Quadrupole

Gas Chromatography coupled with Triple Quadrupole Mass Spectrometry (GC/TQ) offers a robust and sensitive method for
detecting volatile PFAS. However, manual sample preparation could pose a bottleneck, impacting lab productivity. This study presents a robust and fully automated method using the PAL3 platform with GC/TQ for quantifying more than 30 volatile PFAS in FCMs, such as paper coffee cups. The automation approach minimizes human error, improving reliability
and reproducibility for routine analysis and safety assessments of PFAS in FCMs.

ASMS 2025 - Fully Automated Sensitive Quantitation of PFAS in Seafood for Regulatory Screening Using Triple Quadrupole LC/MS

Posters

ASMS 2025 - Fully Automated Sensitive Quantitation of PFAS in Seafood for Regulatory Screening Using Triple Quadrupole LC/MS

Detecting trace PFAS level is challenging due to complex sample preparation like QuEChERS extraction followed by SPE cleanup, and evaporation/reconstitution. The manual steps can be labor-intensive and error-prone, affecting accuracy and reliability. This study developed a fully automated workflow for PFAS quantitation in seafood – shrimp.

Webinars

Advancing PFAS Analysis - Robotic Automation and Streamlined LC/MS Workflows

To support the scientific community and advance PFAS analysis we are proud to offer a webinar on this topic.

Key topics:

PFAS Fundamentals: Learn about the chemistry and significance of PFAS compounds.
Sample Preparation Challenges: Understand current hurdles, regulations and best practices in PFAS extraction and purification.
Automated LC/MS Workflows: Get an overview on automated workflows and see how PAL System integrates with LC/MS to optimize sample preparation and injection.
Deep Dive: Explore online SPE for PFAS analysis.

eBooks

Sample Preparation Compendium

Automated, Green, and Efficient
A Compendium on Micromethods in Analytical Sample Preparation

Discover robust, precise, and reliable analytical methods while following the principles of Green Analytical Chemistry in various fields such as Food & Food Safety, Environmental, Clinical, Life Science, and Chemical.