Derivatization
Derivatization

Repeatability is Essential

Derivatization reactions are the classic sample preparation steps in many chromatographic methods. Manual derivatization usually requires several handling steps, often involving hazardous reagents. PAL Systems provide in-time derivatization in prep-ahead mode right before analysis providing best reproducibility for large sample series, in particular required for metabolomics profiling. 

Derivatization
  • Derivatization procedures for analytes are common for many chromatographic methods. The manual handling is laborious and a time-consuming process. Also, hazardous reagents are involved, and so are critical organic solvents.

  • The PAL System provides workflows for in-time and highly reproducible derivatization reactions. For pracical application there are virtually no limitations for the derivatization chemistry involved. Typical are the silylation, acetylation or methylation reactions for GC/MS, or the dansylchloride or FMOC reactions for LC/MS. 

  • For the most used analytical derivatization in the food industry with the preparation of fatty acid methylesters (FAMEs) both popular reactions with the methylate transesterification and the saponification/BF3 catalyzed methylation are processed on a PAL System with standard workflows.

  • Also, the widely used 2-step derivatization for metabolomic profiling of large sample series in life sciences applications are available as standard workflows. 

  • Derivatization reactions on a PAL System highly benefit from unsurpassed reproducibility due to in-time reaction right before analysis and constant conditions for every sample.

References

Figure 1: Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil

Author: Bradley S. Heater et al
Publication: Biotechnology for Biofuels & Bioproducts
Publisher: Springer Nature
Date: Jun 28, 2019 (http://creativecommons.org/licenses/by/4.0/)

 

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