New PAL System µSPE cartridge for improved reliability

PAL System μ-SPE Cartridges

New PAL System μSPE Cartridges

The PAL µSPE Cartridge stands for a miniaturized and robust SPE technology that allows reliable automation and reduction of used sample and solvent without compromising performance. The µSPE has therefore emerged as a new standard for extraction, purification, concentration or filtration for automated environmentally friendly analysis processes.

The new PAL System µSPE Cartridge consists of two parts. The polymer material used is chemically inert and free from leachables. The outer part (1) provides a higher capacity and flexible volume for filter disks (3) and the sorbent material (4). The bottom outlet is designed to penetrate pre-slit septa and to deliver directly to LC injection ports. The inner plunger (2) provides critical functionality with the compression of the sorbent/filter layers, and a precise needle guide for safe and upright transport. An essential feature is the ability to withstand high pressure and prevent leakage around the syringe needle, which allows for faster loading of larger sample volumes. Applications range from food pesticides, water, and drug analysis to forensic and pharmacological applications.

Key functionality:

Septumless cartridge designs with patented sealing
High pressure rating (up to 10 bar)
Multiple elutions supported
Sorbent capacity: up to 100 mg packing possible
Chemically inert materials used
Free of extractables & leachables
Designed to penetrate pre-slit septa of vials
Designed to elute directly into PAL System liquid injection ports

More about the comparison µSPE to classical SPE

PAL System μSPE Cartridge Selection Guide with Publications

Cartridges	Pesticides Residue Analysis	Application Information	Publications
uSPE-GCQuE1-45-V PAL System μSPE Cartridge GC QuEChERS sorbent mix 1, 45 mg [C18, PSA, MgSO4, GCB] 108 pcs type 01-05B		µSPE Cartridge for GC amenable pesticides. Follows AOAC 2007.01 and EN 15662 methodology.	Steven Lehotay et al, 2022 https://doi.org/10.1016/j.chroma.2022.463596 Amadeo Fernandez-Alba et al. 2023, open Access https://doi.org/10.1016/j.chroma.2023.463906 Mette Poulson et al., 2021, open Access https://doi.org/10.1016/j.chroma.2021.462384 Fully Automated QuEChERS Extraction and Clean-up of Organophosphate Pesticides in Orange Juice, Hübschmann et al, 2023 Performance Qualification Test for PAL System µSPE GC QuEChERS-45-V Cartridges
uSPE-GCQuE2-44-V PAL System μSPE Cartridge GC QuEChERS sorbent mix 2, 44 mg [C18, PSA, MgSO4] 108 pcs type 01-19A		µSPE Cartridge without Carbon for GC amenable pesticides. Follows AOAC 2007.01 and EN 15662 methodology.	Steven Lehotay et al, 2022 https://doi.org/10.1016/j.chroma.2022.463596 Amadeo Fernandez-Alba et al. 2023, open Access https://doi.org/10.1016/j.chroma.2023.463906 Mette Poulson et al., 2021, open Access https://doi.org/10.1016/j.chroma.2021.462384 Fully Automated QuEChERS Extraction and Clean-up of Organophosphate Pesticides in Orange Juice, Hübschmann et al, 2023 Performance Qualification Test for PAL System µSPE GC QuEChERS-45-V Cartridges
uSPE-GCQuE3-45-V PAL System μSPE Cartridge GC QuEChERS sorbent mix 3, 45 mg [PSA, MgSO4] 108 pcs type 01-14A		µSPE Cartridge for GC amenable pesticides in low fat matrices. Follows AOAC 2007.01 and EN 15662 methodology.
uSPE-LC-QuE1-30-T PAL System μSPE Cartridge LC & GC QuEChERS sorbent mix, 30 mg [C18, Z-Sep, GCB] 96 pcs in blister type 01-03A		μSPE Cartridge for pesticides in complex spice matrixes	Goon et al: Journal of AOAC International Vol. 103, No.1, 202 https://doi.org/10.5740/jaoacint.19-0202 Performance Qualification Test for PAL System µSPE LC QuEChERS-30-T Cartridges
uSPE-SAX-50-V PAL System μSPE Cartridge Strong Anion Exchange (SAX), 50 mg for polar, anionic pesticides or other anionic compounds 108 pcs type 01-20A		µSPE Cartridge for LC amenable pesticides. ollows AOAC 2007.01 and EN 15662 methodology.	Analysis of Highly Polar Pesticides - Automated Micro-SPE Clean-up of Complex Food Matrices for LC-MS/MS Florencia Jesús , Amadeo Rodríguez Fernández-Alba, Hans-Joachim Hübschmann

Additional Application Support

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Automated μSPE cleanup for GC/MS or LC/MS analysis of pesticides and environmental contaminants in QuEChERS extracts of foods

Walk away QuEChERS extracts clean-up

See this detailed video of the µSPE workflow for the clean-up of raw pesticides extract. The PAL µSPE Cartridge is operated in scavenger mode eluting the pesticides in a clean extract. The matrix is kept on the sorbent material. The cleaned extract can be injected without waiting time online for LC/MS or GC/MS analysis. All samples are treated on the same time axis for highest reliability.

Advancing PFAS Analysis - Robotic Automation and Streamlined LC/MS Workflows

Webinars

Advancing PFAS Analysis - Robotic Automation and Streamlined LC/MS Workflows

To support the scientific community and advance PFAS analysis we are proud to offer a webinar on this topic.

Key topics:

PFAS Fundamentals: Learn about the chemistry and significance of PFAS compounds.
Sample Preparation Challenges: Understand current hurdles, regulations and best practices in PFAS extraction and purification.
Automated LC/MS Workflows: Get an overview on automated workflows and see how PAL System integrates with LC/MS to optimize sample preparation and injection.
Deep Dive: Explore online SPE for PFAS analysis.

Advantages of automated and miniaturized µSPE clean-up in pesticides residues analysis

Webinars

Advantages of automated and miniaturized µSPE clean-up in pesticides residues analysis

Recent developments in pesticides analysis extend the well-established methods to higher productivity and reliability while minimizing consumption and exposition to harmful chemicals. A workflow was developed that allows automatic preparation of calibration standards, automatic sample dilution, and automatic clean-up of sample extracts on one instrument, showing that miniaturized automated solutions are reducing errors, improving analytical consistency and protecting the environment in one convenient approach.

Presented by:

Thomi Preiswerk, Senior Account Manager, CTC Analytics AG

Gwen Lim , Specialist for automated sample preparation, CTC Analytics

Ederina Ninga, Postdoc, Research Group for Analytical Food Chemistry, Danish Technical University

Fully Automated QuEChERS Extraction and µSPE Clean-up of Organophosphate Pesticides in Orange Juice

Publications

Fully Automated QuEChERS Extraction and µSPE Clean-up of Organophosphate Pesticides in Orange Juice

Chiew Mei Chong1, Hans-Joachim Hübschmann2

1 CTC Analytics Pte. Ltd., Singapore.
2 HANS Analytical Solutions, Osaka, Japan.

Routine Pesticide Analysis using micro-SPE

Application Notes

Routine Pesticide Analysis using micro-SPE

This report presents for the first time the application of µSPE for the clean-up of the raw extracts using ethyl acetate as extraction solvent for pesticides from different also more complex food commodities.

Application Notes

Analysis of Highly Polar Pesticides

Micro-SPE (μSPE) emerged as an automated green micromethod for sample preparation and clean-up in food safety, proteomics, forensic, environmental and analysis since more than ten years. Applications are wideranging and cover drugs, environmental contaminants, and, in particular, the extract clean-up in multiresidue pesticide analysis. The automation of the μSPE sample preparation steps led to the desired standardization of the applied sorbent materials for extract clean-up for the large variety of food commodities, an increase in sample throughput, and the welcome potential for offline and online hyphenation with GC/MS, LC/MS or even IC analysis.

eBooks

QuEChERS Compendium

Automated μSPE cleanup for GC/MS or LC/MS analysis of pesticides and environmental contaminants in QuEChERS extracts of foods

Characterization of the New PAL micro-SPE Cartridge for Pesticides Extract Clean-up

Posters

Characterization of the New PAL micro-SPE Cartridge for Pesticides Extract Clean-up

Since more than ten years micro-SPE (µSPE) emerged as a micromethod for sample preparation and clean-up in food safety, proteomics, forensic, environmental and analysis. Applications are wide-ranging and cover drugs, environmental contaminants, and, in particular, the QuEChERS extract cleanup in multiresidue pesticide analysis. The automation of the µSPE sample preparation steps led to the desired increase in sample throughput, unification of the used sorbent materials for food commodities, and the potential for the online hyphenation with GC/MS and LC/MS instrumentation.

Fully Automated QuEChERS for Organochlorine and Organophosphate Pesticides in Tomato Juice and Red Wine

Posters

Fully Automated QuEChERS for Organochlorine and Organophosphate Pesticides in Tomato Juice and Red Wine

Using PAL Robotic Tool Change (RTC) system, a fully automated QuEChERS was developed for the extraction and clean-up of organochlorine and organophosphate pesticides from homogeneous samples. The automated QuEChERS workflow includes extraction with acetonitrile, salting out with
saturated sodium chloride solution and the clean-up with PAL μSPE prior to injection into the GC-MS/MS for analysis.