Life Science Analysis

Life Science

The automation of applications in life sciences such as proteomics studies, cancer and biomarker studies is very valuable. Typical are the large sample series from cohorts of patients requiring highly reproducible processing, a particular strength of the PAL System.

Temperature controlled sample storage, overlapping and prep-ahead sample preparation, assure that each sample is processed exactly the same way to achieve good reproducibility and comparability of the data. Unattended, fully automated operation of the PAL System is integrated into the chromatographic data systems of all leading manufacturers.

PAL Systems automate a wide range of sample preparation applications in clinical, biotechnological, and biomedical research using dedicated tools and modules with efficient workflows for LC/MS and GC/MS analysis.

Applications include the Bligh & Dyer method, liquid/liquid extraction, protein precipitation and centrifugation steps. For metabolomic profiling the derivatization of analytes is required for high sensitivity analysis.

For therapeutic drug monitoring a dedicated PAL LC/MS Tool offers the direct sampling from primary tubes. An optical sensor is positioned on the sample loop of the tool which monitors the aspiration of blood or serum sample, air gap or bubbles.

Breath gas analysis for volatiles in clinical applications for diagnosis and cancer biomarker research is another fast growing area of Solid Phase Microextraction (SPME) using the rugged SPME Arrows.

The PAL System supports research for the biological activity of proteins by elucidation of their 3-dimensional structure in its active or inactive states. Hydrogen Deuterium Exchange (HDX) is the labeling technique which allows the study of how protein chains are folded during the interaction with their binding partners under physiological conditions.

Workflow Solutions

Discover our and our partners ready-to-go workflows.

Automated Powder Dispensing and Processing

Precise dispensing and weighing technology in an automated workflow.

Proteomics

Automated sample preparation in the proteomics laboratory to optimize throughput and avoid handling errors.

Multiplexing

For a better utilization of the LC/MS.

Reference Literature

Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples
Keywords: phosphoproteome, fractionation, 2D LC
Anal. Chem. 2019 91 (15), 9707-9715
Metabolomic signature of brain cancer
Keywords: adult glioblastoma; magnetic resonance spectroscopy; mass spectrometry; metabolomics; paediatric glioblastom; GC-MS, ITEX Dynamic Headspace
Mol. Carcinog. 2017 Jun 15. doi: 10.1002/mc.22694. [Epub ahead of print]
Compound Property Optimization in Drug Discovery Using Quantitative Surface Sampling Micro Liquid Chromatography with Tandem Mass Spectrometry
Keywords: surface sampling, LC-MS, quantitative tissue distribution, drug discovery
Anal Chem, 2016, 88, 11813-20
Development of a Biocompatible In-Tube Solid-Phase Microextraction Device: A Sensitive Approach for Direct Analysis of Single Drops of Complex Matrixes
Keywords: SPME, biocompatible, complex matrices, in-tube, single drop
Anal Chem, 2016, DOI: 10.1021/acs.analchem.6b03160
Unbiased profiling of volatile organic compounds in the headspace of Allium plants using an in-tube extraction device (OPEN ACCESS)
Keywords: Volatile organic compounds, VOC, GC-TOF–MS, Metabolomics, Headspace, ITEX, Allium
BMC Res Notes, 2016, 9, 133
Proteomics of Cerebrospinal Fluid: Throughput and Robustness Using a Scalable Automated Analysis Pipeline for Biomarker Discovery
Keywords: proteomics, cerebrospinal fluid, CSF, throughput, robustness, RP-LC MS/MS
Anal Chem 2015, 87, 10755-10761
Extended Coverage of Singly and Multiply Phosphorylated Peptides from a Single Titanium Dioxide Microcolumn
Keywords: proteomics, coverage phosphorylated peptides, phospho petides, titanium dioxide microcolum
Anal Chem 2015, 87, 10213-2
An enhanced droplet-based liquid microjunction surface sampling system coupled with HPLC-ESI-MS/MS for spatially resolved
Keywords: droplet-based liquid microjunction surface sampling, complex matrix samples, chemical information from single spot
Anal Bioanal Chem, 2015, 407, 2117-25
Organic Acid Quantitation by NeuCode Methylamidation
Keywords: multiplexed quantitative analysis method, carboxylic acids, liquid chromatography high resolution mass spectrometry, LC-MS
Anal Chem, 2014, 86, 4402-4408
Time Window Expansion for HDX Analysis of an Intrinsically Disordered Protein.
J Am Soc Mass Spectrom 2013; 24(10): 1584-92
Quantitative Metabolite Profiling Utilizing Parallel Column Analysis for Simultaneous Reversed-Phase and Hydrophilic Interaction Liquid Chromatography Separations Combined with Tandem Mass Spectrometry
Keywords: metabolomics, profiling, orthogonal separation, column switching
Anal Chem, 2014, 86, 4145-4150
Method Development for Fecal Lipidomics Profiling (OPEN ACCESS)
Keywords: Fecal analysis, lipidomics, mass spectrometry, liquid chormatography, high pressure cycling LC/MS, premature infant, neonate
Anal Chem, 2013, 85, 1114−1123
Evaluation of a novel commercial assay for the determination of cyclosporine A, tacrolimus, sirolimus and everolimus by liquid chromatography-tandem mass spectrometric assay
Keywords: immunosuppressant, blood samples, in-vitro diagnostic (IVD)- mass spectrometric immunosuppressant assay, LC/MS
Ther Drug Monit, 2013, 35, 129-32
Rapid Screening for Potential Epitopes Reactive with a Polycolonal Antibody by Solution-Phase H/D Exchange Monitored by FT-ICR Mass Spectrometry
J. Am. Soc. Mass Spectrom. (2013) 24;1016-1025
Granger causality in integrated GC–MS and LC–MS metabolomics data reveals the interface of primary and secondary metabolism
Metabolomics (2013) 9: 564–574
Improved Protein Hydrogen/Deuterium Exchange Mass Spectrometry Platform with Fully Automated Data Processing
Anal. Chem. 2012, 84, 4942−4949
Determination of paracetamol in mouse, rat and dog plasma samples by laser diode thermal desorption–APCI-MS/MS
Bioanalysis (2012) 4(11), 1327–1335
Proteomic Profiling of Thyroid Papillary Carcinoma
J Thyroid Res. 2012;2012:815079
High-throughput flow injection analysis of labeled peptides in cellular samples – ICP-MS analysis versus fluorescence based detection
Int J Mass Spectrom. 2011, 307, 105–111
Methods for the Analysis of High Precision Differential Hydrogen Deuterium Exchange Data
Int J Mass Spectrom. 2011; 302(1-3): 59–68
Drug-excipient compatibility testing—Identification and characterization of degradation products of phenylephrine in several pharmaceutical formulations against the common cold
J Pharm Biomed Anal 55 (2011) 949–956
Structural characterization of both the non-proteolytic and proteolytic activation pathways of coagulation Factor XIII studied by hydrogen–deuterium exchange mass spectrometry
Int. J. Mass Spec, 302, 2011, 139–148
Strategy of Accelerated Method Development for High-Throughput Bioanalytical Assays Using Ultra High-Performance Liquid Chromatography Coupled with Mass Spectrometry
Anal. Chem. 2009, 81, 9225–9232
Comprehensive Analytical Strategy for Biomarker Identification based on Liquid Chromatography Coupled to Mass Spectrometry and New Candidate Confirmation Tools
Anal. Chem. 2009, 81, 7677–7694
TopCount coupled to ultra-performance liquid chromatography for the profiling of radiolabeled drug metabolites in complex biological samples
Keywords: Scintillation counter, radiolabeling, UHPLC
J Chromatogr B Analyt Technol Biomed Life Sci. 2006; 844(1):96-103
Cherry-picking in an orchard: unattended LC/MS analysis from an autosampler with > 32,000 samples online
Keywords: High throughput screening, LC/MS
J Biomol Screen. 2006 April,11 (3): 318-22. EPub 2006 Feb 20.
Enantiomeric separation and quantification of pindolol in human plasma by chiral liquid chromatography/tandem mass spectrometry using staggered injection with a CTC Trio Valve System
Keywords: High throughput screening, LC/MS
Rapid Commun Mass Spectrom. 2006; 20(2): 291-7
Description and Validation of a Four-Channel Staggered LC-MS/MS System for High-Throughput in Vitro Screens
Keywords: High throughput Screening, LC/MS
Anal. Chem. 2005, 77, 1905-1910
SpeedScreen: The “Missing Link” between Genomics and Lead Discovery
Keywords: High throughput screening, LC/MS
J Biomolecular Screening 2004: 9, 498-505
Headspace solid-phase microextraction and gas chromatography-mass spectrometry for the determination of 5alpha-androst-2-en-17-one and -17beta-ol in the female Asian elephant: application for reproductive monitoring and prediction of parturition
Keywords: SPME, GC-MS
J Steroid Biochem Mol Biol. 2003 Feb; 81(2-3):383-91
Derivatization of Ehinylestradiol with Dansyl Chloride To Enhance Electrospray Ionization: Application in Trace Analysis of Ethyinylestradiol in Rhesus Monkey Plasma
Keywords: Ethinylestradiol, derivatization dansyl chloride, LC/MS
Anal Chem. 2002, 74(16), 4136-44

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