Automated Sample Preparation with PAL System
The Key to Reliable LC/MS and GC/MS Data
The increasing sensitivity of modern mass spectrometry (MS) instruments demands standardized and automated sample preparation to ensure the integrity of analytical data [1]. PAL System offers a robust solution by automating critical sample preparation steps, thereby significantly minimizing human error and maximizing data quality for both liquid chromatography/mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC/MS) workflows. This automation is crucial for obtaining reliable results, especially in demanding fields like metabolomics, proteomics, and clinical diagnostics [2]. Our robotic platform can be used as a stand-alone sample handler, integrated in all major Chromatography Data Systems (CDS), or can be built into other products (see our OEM partnerships).
Mastering Complexity from Liquid Handling to Micromethods
The PAL System offers a comprehensive and automated suite of sample preparation techniques, seamlessly integrating both well-established methodologies and innovative micromethods to address a wide spectrum of analytical challenges. This commitment extends to supporting Green Analytical Chemistry principles by significantly minimizing solvent consumption and waste generation [9], [10].
uSPE (Micro-Solid Phase Extraction): A miniaturized and automated form of Solid Phase Extraction (SPE), uSPE is ideal for high-throughput analysis and reduced solvent usage. The PAL System precisely integrates uSPE cartridges, enabling efficient sample clean-up and analyte enrichment, offering significant advantages over traditional SPE methods [6]. Explore more about uSPE
SPME (Solid Phase Microextraction): This solvent-free extraction technique is particularly well-suited for the analysis of volatile and semi-volatile organic compounds [8]. The PAL System automates SPME using both immersive and headspace sampling modes, providing exceptional versatility for diverse applications. Furthermore, it supports the advanced SPME Arrow technology, known for its enhanced robustness and sensitivity [11], [12]. Discover more on SPME Arrow
ITEX (In-Tube Extraction): An active and dynamic headspace sampling technique, ITEX allows for the efficient enrichment of volatile organic compounds (VOCs) [13]. The PAL System's automation of ITEX significantly enhances extraction efficiency and lowers detection limits, crucial for trace-level analysis. More on ITEX
Automated Liquid-Liquid Extraction (LLE): The PAL System automates the classic Liquid-Liquid Extraction (LLE) method, expertly separating analytes based on their differential solubility in immiscible solvents. Automation with the PAL System minimizes manual handling, reduces the risk of errors, and significantly improves reproducibility.
Automated Solid-Liquid Extraction (SLE): This fundamental extraction technique, where target analytes are extracted from a solid matrix using a suitable solvent, is seamlessly automated by the PAL System. This automation ensures enhanced efficiency and consistency compared to traditional manual SLE procedures. Learn more about LLE and SLE
QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe): Originally developed for pesticide residue analysis, QuEChERS has evolved into a versatile approach for extracting a broad range of analytes from various complex matrices. The PAL System automates and can miniaturize the QuEChERS method, facilitating µSPE applications and further aligning with Green Chemistry principles. How to use QuEChERS
And More: The PAL System's modular architecture allows for the integration of a wide array of additional tools and accessories, including centrifuges, agitators, syringe tools, and specialized modules. This adaptability enables the automation of virtually any sample preparation workflow, ensuring the system can evolve with your analytical needs. The possibilities are truly limitless! Explore all PAL System types!
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[2] W. B. Dunn et al., “Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry,” Nat Protoc, vol. 6, no. 7, pp. 1060–1083, Jun. 2011, doi: 10.1038/nprot.2011.335.
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[13] D. Michiu, M. Tofană, S. A. Socaci, E. Mudura, L. C. Salanță, and A. C. Fărcaş, “Optimization of ITEX/GC-MS method for beer wort volatile compounds characterisation,” 2012. [Online]. Available: http://
